human primary retinal pigment epithelial cells  (ATCC)

Journal: Scientific Reports

Article Title: Thermal power plant proximity alters Olive composition and induces cytotoxicity in human cells

doi: 10.1038/s41598-025-18066-y

Figure Lengend Snippet: The cell viability of ARPE-19 human retinal epithelial cells treated with fruit (up panel) and leaf (down panel) extracts at varying concentrations for 24 h and 48 h. Extracts were obtained from olives grown at different distances from the Yatağan TPP, Deştin Village (farthest location), Central (middle location), and Thermal (Şahinler Village - closest location). Values represent mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: The cells included MCF10A (ATCC, CRL-10317TM) human mammary gland epithelial cells, ARPE-19 (ATCC, CRL-2302TM) human eye retinal pigment epithelial cells, HUVEC (ATCC, CRL-1730TM) primary human umbilical vein endothelial cells, and BEAS-2B (ATCC, CRL-3588TM) epithelial cells isolated from normal human bronchial epithelium.

Techniques:

Journal: Scientific Reports

Article Title: Thermal power plant proximity alters Olive composition and induces cytotoxicity in human cells

doi: 10.1038/s41598-025-18066-y

Figure Lengend Snippet: The cell viability of MCF10A human breast epithelial cells treated with fruit (up panel) and leaf (down panel) extracts at varying concentrations for 24 h and 48 h. Extracts were obtained from olives grown at different distances from the Yatağan TPP, Deştin Village (farthest location), Central (middle location), and Thermal (Şahinler Village - closest location). Values represent mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: The cells included MCF10A (ATCC, CRL-10317TM) human mammary gland epithelial cells, ARPE-19 (ATCC, CRL-2302TM) human eye retinal pigment epithelial cells, HUVEC (ATCC, CRL-1730TM) primary human umbilical vein endothelial cells, and BEAS-2B (ATCC, CRL-3588TM) epithelial cells isolated from normal human bronchial epithelium.

Techniques:

Journal: Scientific Reports

Article Title: Thermal power plant proximity alters Olive composition and induces cytotoxicity in human cells

doi: 10.1038/s41598-025-18066-y

Figure Lengend Snippet: The cell viability of BEAS-2B human bronchial epithelial cells treated with fruit (up panel) and leaf (down panel) extracts at varying concentrations for 24 h and 48 h. Extracts were obtained from olives grown at different distances from the Yatağan TPP, Deştin Village (farthest location), Central (middle location), and Thermal (Şahinler Village - closest location). Representative cells after the MTT assay are given after leaf extracts for 48 h (images taken by 5x objective of Zeiss AxioVert inverted microscope). Values represent mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: The cells included MCF10A (ATCC, CRL-10317TM) human mammary gland epithelial cells, ARPE-19 (ATCC, CRL-2302TM) human eye retinal pigment epithelial cells, HUVEC (ATCC, CRL-1730TM) primary human umbilical vein endothelial cells, and BEAS-2B (ATCC, CRL-3588TM) epithelial cells isolated from normal human bronchial epithelium.

Techniques: MTT Assay, Inverted Microscopy

Journal: bioRxiv

Article Title: Multi-scale Molecular Imaging of Human Cells reveals COPI and COPII Vesicles at ER Exit Sites

doi: 10.1101/2025.07.29.667472

Figure Lengend Snippet: A. Wild type RPE1 cells immunostained against Sec31A. B. Scatter plot of number of Sec31A positive structures in wild type RPE1 cells, colour coded by experimental repeat, n = 32 cells. C. HaloTag-Sec23A-RPE1 cells dyed with JFX650-HaloTag ligand (green) and co-stained using an antibody directed against Sec31A (purple). Inset highlights tight colocalization of HaloTag-Sec23A fusion protein and endogenous Sec31A. D. Example cryo-fluorescence wide field image of HaloTag-Sec23A-RPE1 cells grown on grids stained with Oregon Green HaloTag ligand before and after milling. HaloTag-Sec23A displays a punctate staining pattern throughout the cytoplasm and is concentrated at the perinuclear region. The red box in the left panel indicates the digitally magnified region in the other panels which show cryo-fluorescence before (middle panel) and after (right panel) milling. Four cells are indicated by green dotted outlines. Blue arrow heads indicate clusters of fluorescent fiducials. Green arrows indicate fluorescent puncta visible both before and after milling. Scale bars: 10um, inset in panel C: 1um.

Article Snippet: Wild type human retinal pigmented epithelial cells (RPE-1) were obtained from ATCC and were cultured in DMEM/F12 (ThermoFisher Scientific) plus 10% FBS, L-glutamine and 1% Pen/Strep (Bio Basic).

Techniques: Staining, Fluorescence